Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves

Table 1 Candidate reference genes and primers sequences used for real-time-qPCR

Gene names	Gene description	Primer 5’-3’	Correlation coefficient (R²)	qRT-PCR efficiency (%)	Annealing temperature (C^º)	Accession number
eEF-1α	Eukaryotic elongation factor 1-alpha	TTTCACTCTTGGTGTGAAGCAGAT GACTTCCTTCACGATTTCATCGTAA	0.999	99.9	61–61	gi\|351,734,545\|
UBE2D2	Ubiquitin-conjugating enzyme E2	ATTGCCTGCTGATCCTGATCTGC ACCACTGCAACCACACCAAGC	0.945	92	60–61	MT822516
β-tubulin	Beta-tubulin	GCTGACCACACCTAGCTTTGG AGGGAACCTTAGGCAGCATGT	0.986	98.9	60–60	MT822512
Actin 11	Actin 11	CAGCCACACTGTCCCCATCTA AGCAAGGTCGAGACGAAGGA	0.989	99.5	60–60	gi\|255,684,847\|
HSP70	Heat shock protein	CTTGGTGCGTCAGCGTATCT GCGGTGCCATTGTGTTTCAT	0.989	96.5	61 − 60	NM_001357341.1
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	AAGCCAGCATCCTATGATCAGATT CGTAACCCAGAATACCCTTGAGTTT	0.994	105.3	61–61	MT822517
UbcH10	Ubiquitin 10	TGGTCAGTAATCAGCCAGTTTGG GCACCACAAATACTTGACGAACAG	0.970	103.3	61–61	gi\|351,727,992\|
eIF4A	Eukaryotic initiation factor 4a	CATGGATGTACCTGTGGTGAAAC CTGTCAGCAGAAGGTCCTCATTA	0.999	105.5	60–61	AK073620
25 S rRNA	25 S ribosomal RNA	AAGGCCGAAGAGGAGAAAGGT CGTCCCTTAGGATCGGCTTAC	0.980	96	60–60	AK119809
18 S rRNA	18 S ribosomal RNA	CTACCACATCCAAGGAAG CAATTACCAGACACTAACG	0.997	98.7	61 − 60	gi\|343,347\|
SSR	Sterol side chain reductase	AGGTGGGAGATATGCTAGAATG CATTCTGTGTGTCTCCCTGCC	-	-	61 − 60	MK060115.1

ISSN: 1746-4811