Fig. 5

Determination of the optimal conditions for thylakoid extraction and separation of photosynthetic complexes from P. oceanica. A CN-PAGE of solubilized membrane from 26 m depth plants extracted with different concentrations of VitC (0–10%) in the presence of 5% PEG. The membranes were solubilized using a mixture of 1% β-DDM + 1% digitonin and 5 of μg chlorophyll was loaded onto the gel. B CN-PAGE of solubilized membrane extracted from 26 m depth plants with different concentrations of PEG (0.1–5%) in the presence of 5% VitC. The membranes were solubilized using a mixture of 1% β-DDM + 1% digitonin and 5 μg of chlorophyll was loaded onto the gel. C CN-PAGE vs BN-PAGE of 2% α-DDM solubilized membranes from 26 m depth plants. The thylakoid membranes were extracted using the optimized PVC protocol (5% PEG and 5% VitC)