Fig. 4

SDS-PAGE and immunoblot analyses of Posidonia oceanica leaf proteins from samples collected at different depths. Coomassie-blue-stained SDS-PAGE gels of total proteins extracted from P. oceanica leaves using laemmli denaturing buffers containing VitC (5%) without PEG (A) or with PEG (5%) (B). C Immunoblot analysis of photosynthetic proteins extracted from leaves in the presence of vitamin C (5%) and PEG (5%). Extraction was carried out using equal amounts of leaf material ground in denaturing buffer and equal volumes of protein extracts were loaded on the gels. For immunoblot analyses, equal protein amounts were loaded based on quantity evaluation from the scanning of Coomassie-blue stained gel