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Table 3 LC-MS/MS method reproducibility test

From: Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry

Analyte

Reproducibility (n = 3, %RSD)

Low

Medium

High

ACC

0.19

0.12

0.75

tZ

0.92

1.31

8.05

cZ

1.09

0.59

8.75

iP

1.45

2.99

5.84

tZR

4.00

2.02

7.67

cZR

0.66

5.21

7.92

iPR

1.70

1.48

10.03

IAA

1.28

1.09

6.81

oxIAA

1.21

2.74

8.44

IAA-Glu

0.56

0.47

10.23

IAA-Asp

2.05

2.09

9.19

ABA

4.25

5.89

14.69

JA

0.58

2.89

13.18

JA-Ile

5.66

2.00

5.78

SA

4.40

2.35

8.41

  1. The reproducibility test was performed by injecting three different concentrations (low, medium and high) of standard mixtures in three days from the same sample vial. The amounts (pmol per injection) for each compound (low; medium; high) were as following: ACC, JA-Ile (0.045; 0.45; 4.5); tZ, cZ, iP, tZR, cZR, iPR (0.005; 0.05; 0.5); IAA, oxIAA, IAA-Glu, IAA-Asp, ABA (0.45; 4.5; 45); JA and SA (0.225; 2.25; 22.5). Results in %RSD difference between normalized peak area, n = 3
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Plant Methods

ISSN: 1746-4811