Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry

Table 2 Method validation parameter summary (for detailed description see „method Validation“chapter)

Analyte	Spiked content (pmol)	Average Accuracy (%bias)	Average Precision (%RSD)	Average Process Efficiency (%)	Average Matrix Effect (%)	Average Recovery (%)
ACC	2-200	5.59	3.2	21.01	23.74	88.17
tZ	0.01–0.1	10.02	1.81	44.92	58.56	90.92
cZ	0.01-1	8.92	0.84	42.66	63.98	68.18
iP	0.01-1	6.12	2.71	39.12	49.19	80.4
tZR	0.01-1	12.3	2.44	39.76	45.74	87.53
cZR	1	12.16	2.05	62.43	75.53	82.65
iPR	0.01-1	12.65	1.94	56.12	83.94	65.1
IAA	0.1–100	12.72	1.63	50.38	80.08	63.53
oxIAA	10–100	11.81	1.82	44.93	59.88	75.17
IAA-Glu	0.1–100	8.63	3.2	77.57	95.95	81.71
IAA-Asp	0.1–100	7.48	2.06	66.13	81.82	80.95
ABA	0.1–100	10.39	1.62	72.15	106.27	69.85
JA	0.1–100	8.31	2.48	68.21	96.72	70.76
JA-Ile	1	6.98	4.95	17.69	48.12	36.74
SA	0.5–50	9.31	1.87	106.88	133.62	79.94

Values for Average Process Efficiency, Average Matrix Effect and Average Recovery are means ± SD; n = 4. Accuracy and precision were estimated by spiking 10 mg of FW A. thaliana with authentic standards (n = 4)

ISSN: 1746-4811