Table 1 Optimized LC-MS/MS parameters for analyzed compounds and their corresponding internal standards

ACC

6.96

272.1

171.0

[M + H]⁺

18

0.00045–4.5

0.9995

[²H₄]-ACC

[²H₄]-ACC

6.89

276.1

171.0

[M + H]⁺

18

-

-

tZ

4.67

220.2

136.1

[M + H]⁺

19

0.00036–0.36

0.9996

[¹³C₅]-tZ

cZ

5.24

220.2

136.1

[M + H]⁺

19

0.00036–0.36

0.9994

[¹³C₅]-cZ

iP

10.3

204.2

136.0

[M + H]⁺

12

0.001–1

0.9982

[²H₆]-iP

tZR

8.57

352.1

220.1

[M + H]⁺

20

0.0001–0.5

0.9991

[²H₅]-tZR

cZR

9.19

352.1

220.1

[M + H]⁺

20

0.001–1

0.9988

[²H₅]-tZR

iPR

15.85

336.1

204.1

[M + H]⁺

20

0.001–1

0.9997

[²H₆]-iPR

[¹³C₅]-tZ

4.66

225.1

141.1

[M + H]⁺

20

-

-

-

[¹³C₅]-cZ

5.23

225.1

141.1

[M + H]⁺

20

-

-

-

[²H₆]-iP

10.16

210.0

137.0

[M + H]⁺

12

-

-

-

[²H₅]-tZR

8.45

357.1

225.1

[M + H]⁺

20

-

-

-

[²H₆]-iPR

15.62

342.1

210.1

[M + H]⁺

20

-

-

-

IAA

11.65

176.1

130.1

[M + H]⁺

24

0.018–36

0.9991

[¹³C₆]-IAA

oxIAA

7.68

192.1

146.0

[M + H]⁺

12

0.009–90

0.9993

[¹³C₆]-oxIAA

IAA-Glu

9.98

305.2

130.1

[M + H]⁺

24

0.09–90

0.9990

[¹³C₆]-IAA-Glu

IAA-Asp

8.25

291.1

130.1

[M + H]⁺

36

0.0045–90

0.9995

[¹³C₆]-IAA-Asp

[¹³C₆]-IAA

11.64

182.1

136.0

[M + H]⁺

24

-

-

-

[¹³C₆]-oxIAA

7.67

198.1

152.1

[M + H]⁺

12

-

-

-

[¹³C₆]-IAA-Glu

9.96

311.2

136.1

[M + H]⁺

24

-

-

-

[¹³C₆]-IAA-Asp

8.22

297.1

136.0

[M + H]⁺

36

-

-

-

ABA

17.89

263.2

153.1

[M + H]⁻

8

0.0018–3.6

0.9992

[²H₆]-ABA

JA

18.19

209.2

58.8

[M + H]⁻

8

0.0018–18

0.9996

[²H₆]-JA

JA-Ile

18.85

324.3

151.2

[M + H]⁺

16

0.0018–3.6

0.9986

[²H₂]-JA-Ile

SA

8.14

137.1

92.8

[M + H⁻]

16

0.045–45

0.9988

[²H₄]-SA

[²H₆]-ABA

17.87

269.2

159.1

[M + H]⁻

8

-

-

-

[²H₆]-JA

18.17

215.2

58.8

[M + H]⁻

8

-

-

-

[²H₂]-JA-Ile

18.84

326.3

151.2

[M + H]⁺

16

-

-

-

[²H₄]-SA

8.03

141.1

96.8

[M + H]⁻

16

-

-

-