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Fig. 6 | Plant Methods

Fig. 6

From: Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR

Fig. 6

A Chromosome in situ hybridisation on two root segments from each of two wheat × barley hybrids (plant No. 6 and 13 on Fig. 4). The barley genome is detected by GISH (red label) and the barley chromosomes are identified by FISH (5 S rDNA, green label). The chromosomes are counterstained with DAPI (blue). Bars = 10 μm. B Schematic position of the 5 S rDNA-specific probe on the barley genome (red line, position of the centromere). C MPCR amplification of chromosomes 1–7 of the A, B, D, and H sub-genomes of wheat × barley hybrids (same plant Nos. 6 and 13) on DNA templates extracted from the root segments used for GISH (Fig. 5A). M – Molecular size marker (GeneRuler™ 100 bp plus DNA Ladder), GP – ‘Golden Promise’, CS – ‘Chinese Spring’, DW – no-template control. Reaction components: Phusion Green HF Buffer and Phusion Hot Start II High-Fidelity DNA Polymerase

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Plant Methods

ISSN: 1746-4811