Fig. 5From: Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCRMPCR amplification of chromosomes 1–7 of the A, B, D, and H sub-genomes of wheat × barley hybrids (1–16). M – Molecular size marker (GeneRuler™ 100 bp Plus DNA Ladder), GP – ‘Golden Promise’, CS – ‘Chinese Spring’, DW – no template control. Reaction components: Phusion Green HF Buffer and Phusion Hot Start II High-Fidelity DNA PolymeraseBack to article page