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Fig. 4 | Plant Methods

Fig. 4

From: Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR

Fig. 4

MPCR amplification of chromosomes 1–7 of the A, B, and D sub-genomes of different wheat cultivars (1: Chinese Spring reference genome, 2: Bobwhite, 3: Fielder, 4: Bánkúti 1201, 5: LRPB Lancer, 6: CDC Stanley, 7: Paragon, 8: SY Mattis, 9: Julius, 10: Cadenza, 11: Weebill 1, 12: Claire, 13: Robigus, 14: Jagger) and the H genome of five barley cultivars (15: Golden Promise reference genome, 16: Morex, 17: Igri, 18: California Mariout, 19: Esperanza). Arrow – increased 5 A-specific product sizes. M – Molecular size marker (GeneRuler™ 100 bp Plus DNA Ladder). Reaction components: Phusion Green HF Buffer and Phusion Hot Start II High-Fidelity DNA Polymerase

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Plant Methods

ISSN: 1746-4811