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Fig. 3 | Plant Methods

Fig. 3

From: Rapid and cost-effective molecular karyotyping in wheat, barley, and their cross-progeny by chromosome-specific multiplex PCR

Fig. 3

A Single PCR amplifications with primers specific to chromosomes 1–7 of the A, B, and D sub-genomes of wheat (‘Chinese Spring’) and the H genome of barley (‘Golden Promise’). 1–7: the PCR amplicons corresponding to each chromosome, M – Molecular size marker (GeneRuler™ 100 bp Plus DNA Ladder). Reaction components: Phusion Green HF buffer and Phusion Hot Start II High-Fidelity DNA Polymerase. B MPCR amplification of chromosomes 1–7 of the A, B, and D sub-genomes of wheat (‘Chinese Spring’) and the H genome of barley (‘Golden Promise’) using different buffers and DNA polymerases. 1: Phusion U Green Multiplex PCR Master Mix, 2: Phusion Green HF Buffer using Phusion Hot Start II High-Fidelity DNA Polymerase, 3: Phire Green HF buffer using Phire Hot Start II High-Fidelity DNA Polymerase, 4: Phusion Green HF buffer using Phire Hot Start II High-Fidelity DNA Polymerase. M – Molecular size marker (GeneRuler™ 100 bp Plus DNA Ladder)

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Plant Methods

ISSN: 1746-4811