A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants

Table 1 Optimization of cotyledon-VIGS of CrChlH in C. roseus using different time (days) and varying Agrobacterium concentration (OD₆₀₀).

Optimization Parameter (day-OD₆₀₀)	Yellow cotyledon efficiency*
Set 1
2d-1.0	3/50
3d-1.0	13/50
4d-1.0	24/50
5d-1.0	42/50
Set 2
5d-0.2	26/50
5d-0.5	50/50
5d-1.0	37/50
5d-2.0	28/50

*The efficiency is shown as the number of yellow cotyledons per 50 cotyledons. Data presented here are from three biological replicates. For each biological replicate, all cotyledons from 9 seedlings (18 cotyledons/replicate) were pooled to determine the phenotype

ISSN: 1746-4811