Fig. 4

Cotyledon-VIGS of CrMYC2 with or without MeJA treatment. Relative expression of CrMYC2, ORCA2, and ORCA3 in empty vector control (TRV) and CrMYC2-VIGS (TRV-CrMYC2) cotyledons without (a) or with (b) MeJA treatment (+ MeJA; 100 µM). MeJA (100 µM) was added to the petri dishes containing the seedlings and petri dishes covered with the lids for 2 h. Gene expression was measured using RT-qPCR. The C. roseus RPS9 gene was used as an internal reference gene. The values represent means ± SD from three biological replicates. For each biological replicate, entire cotyledons were pooled from 8–9 seedlings (16–17 cotyledons). Statistical significance was calculated using Student’s t test (** P < 0.01)