Fig. 2

Cotyledon-VIGS of CrChlH. Phenotypes of the empty vector (TRV) control (a) and CrChlH-VIGS (TRV-CrChlH) (b) seedlings show the yellow cotyledons of CrChlH-VIGS seedlings. (c) Relative expression of CrChlH in control (TRV) and CrChlH-VIGS (TRV-CrChlH) cotyledons. (d) Concentrations of chlorophylls a (Chl a) and b (Chl b) in control (black) and CrChlH-VIGS (gray) cotyledons. Inserted picture shows the color difference of the chlorophyll solutions in the control (left) and TRV-CrChlH (right). CrChlH expression was measured using RT-qPCR. The C. roseus RPS9 gene was used as an internal reference gene. The values represent means ± SD from three biological replicates. For each biological replicate, entire cotyledons were pooled from 8–9 seedlings (16–17 cotyledons). Statistical significance was calculated using Student’s t test (** P < 0.01)