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Table 2 Embryo rescue in A. axillaris hairy root-derived T1 seeds

From: Injection-based hairy root induction and plant regeneration techniques in Brassicaceae

ER media

GA3 [mg/L]

BAP [mg/L]

IAA [mg/L]

Charcoal [%]

Germination rate [%]

ERM1

0.5

–

–

0.4

39 ± 3

ERM2

0.5

1

–

0.4

41 ± 5

ERM3

0.5

–

1

0.4

42 ± 5

ERM4

0.5

1

1

0.4

66 ± 4

Growing media

GA3 [mg/L]

BAP [mg/L]

IBA [mg/L]

Survival rate [%] (n)

 

MS + B5

–

–

–

9 (2/23)

 

SEM

0.03

0.5

–

53 (18/34)

 

RIM

–

–

0.5

37 (14/38)

 
  1. 3–4 independent ER assays were performed with 11–14 seeds per assay in different embryo-rescue media (ERM). For details, see Methods and Results. The germinating seeds were transferred to cultivation boxes with either medium without hormone, shoot elongation medium (SEM), or root inducing medium (RIM). The survival rate was calculated two months after the transfer
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Plant Methods

ISSN: 1746-4811