Fig. 2
From: Injection-based hairy root induction and plant regeneration techniques in Brassicaceae
Process of hairy root induction and regeneration in A. axillaris. Hairy roots were induced by injection with Agrobacterium carrying a Ri plasmid. Selected hairy root lines were cultivated on Petri dishes. The shoot formation was elicited by culturing hairy roots on the regeneration medium. The shoots were subsequently transferred to the shoot elongation medium (SEM) and the root induction medium (RIM). Rooted plants were transferred to the soil and produced T1 hairy root-derived seeds. Alternatively, regenerants on RIM were subjected to another injection-based transformation with Agrobacterium carrying both the Ri plasmid and a binary vector expressing a nuclear-targeted fluorescent protein VENUS (VENUS-NLS) and carrying a kanamycin resistance cassette. Emerging hairy root lines were selected on kanamycin and screened for fluorescent signal