Fig. 6

Evolution of antimicrobial activity during germination and seedling growth. A Experimental design used to release dormancy and produce exudates after germination (5 d) and seedling growth (10 d). The rolled-up arrow corresponds to the replacement of the exudate by fresh water. B, C. Antimicrobial activity of exudates during germination seeds (B) and during seedling growth (C) of H10-205, H10-131 and Cervil genotypes against A. brassicicola growth calibrated at 10³ CFU/mL. D. Antimicrobial activity from seed exudate during germination and seedling growth against A. brassicicola calibrated at 10⁴ CFU/mL. Data are expressed as the normalized growth ratio between the AUC with and without exudate. Points in the box plots corresponds of the three technical replicates per measurement. Measurements of antimicrobial activity of the exudate from H10-205, H10-131 and Cervil genotype were repeated respectively seven, three and five times in the panel B; respectively four, one and two times in the panel C; and one time for all genotype in the panel D. The dashed line corresponds to control growth without exudate. The star indicates a significant difference from control (t-test or Mann–Whitney test, p < 0.05). Different letters indicate a significant difference between genotypes (Kruskal–Wallis test, Dunn method, p < 0.05). G (%), germination percentage was determined on 60 seeds