Fig. 5

Influence of primary and secondary dormancy on antimicrobial activity. A Experimental design used to produce the exudate. Thermodormancy (TD) was induced by a 5 day imbibition at 35 ℃ in the dark using non dormant seeds. Exudates were collected after 5 d of imbibition, seeds were transferred into a fresh water solution and exudate was collected after another 5 d of incubation at 20 ℃. The rolled-up arrow corresponds to the replacement of the exudate by fresh water. B-C. Normalized growth of A. brassicicola calibrated at 10³ CFU/mL of primary dormant (PD) and thermodormant seeds of the Cervil (B) and H10-131 (C) genotype after 0–5 and 5–10 d of incubation. Data are expressed as the normalized growth ratio between the AUC with and without exudate. Points in the box plots corresponds of the three technical replicates per biological replicate (n). n = 8 for TD Cervil at 35 ℃; n = 6 for TD Cervil at 20 ℃ and n = 3 for the others conditions. The dashed line corresponds to control growth without exudate. A star indicates a significant difference from control (t-test or Mann–Whitney test, α = 0.05). Different letters indicate a significant difference between conditions from Cervil genotype (Kruskal–Wallis test, Dunn method, p < 0.05) and H10-131 genotype (ANOVA, Holm-Sidak method, p < 0.05). G (%), germination percentage was determined on three replicates of 60 seeds (± se)