Fig. 4
From: A method to determine antifungal activity in seed exudates by nephelometry
Dynamics of antimicrobial activity during imbibition of primary dormant seeds. A The effect of imbibition time when Cervil seed exudate is collected on the growth of A. brassicicola at 103 CFU/mL. Data represent the average of three technical replicates ± SD and are fitted with a sigmoidal regression as an aid to the eye. B, C. The impact of the genotypes on growth of A. brassicicola at 103 CFU assessed after 3 (B) and 10 day (C) of imbibition before collecting the exudate. Data are expressed as the normalized growth ratio between the AUC with and without exudate. Points in the box plots corresponds to three technical replicates per biological replicate (n). n = 1 for data in the panel B. In the panel C, n = 1 for H10-165, H10-131 and MT-209; n = 2 for H10-205 and Cervil. The dashed line corresponds to control growth without exudate. A star indicates a significant difference from control (t-test or Mann–Whitney test, p < 0.05). G (%), germination percentage was determined on 60 seeds