Fig. 2

Modulation of assay sensitivity the detection of antimicrobial activity from exudates of primary dormant Cervil seeds. A Growth curve of A. brassicicola at 10⁴ and 10³ CFU/mL with and without (control) exudates. Data represent the average of three technical replicates ± SD. B Effect of conidia concentration on antimicrobial activity from exudates. Data are expressed as the normalized growth ratio between the AUC with and without exudate. Box plots represent the median of two replicates. Points in the box plots corresponds of the three technical replicates per biological replicates. C The effect of seed number used to produce exudate on the growth of A. brassicicola at 10³ CFU/mL. Data points are expressed as the normalized growth ratio between the AUC with and without exudate and represent the mean of three replicates ± SD. D Dose–response curve of exudate dilution on the fungal growth calibrated at 10³ CFU/mL. Data points represent the average of three technical replicates ± SD. Data were fitted with a sigmoidal regression as an aid to the eye. The dashed line corresponds to control growth without exudate. A star indicates a significant difference from control without exudate (Mann–Whitney test, p < 0.05). Different letters indicate a significant difference between conditions (Kruskal–Wallis test, Dunn method, p < 0.05). AUC, area under the curve; RNU, relative nephelometry units