Table 2 Mutation rates in the transformed protoplasts, quantified by targeted deep sequencing
From: Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex
Gene | sgRNAs | Total reads | Indel | Indel frequency (%) |
|---|---|---|---|---|
Control | – | 32,223 | 0 | 0 |
DcACO1 | 1 | 45,946 | 4044 | 8.80 |
2 | 35,360 | 3809 | 10.80 | |
Control | – | 221,036 | 0 | 0 |
DcACS1 | 1 | 40,405 | 23,637 | 58.50 |
2 | 259,713 | 6337 | 2.44 | |
3 | 201,000 | 402 | 0.20 | |
4 | 229,641 | 44,252 | 19.27 | |
5 | 120,779 | 21,547 | 17.84 |