Table 1 In vitro cleavage efficiency of sgRNAs targeting the DcACO1 and DcACS1 gene loci
From: Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex
Gene | sgRNAs | PCR Amplicon (bp) | Cleaved fragments (bp) |
|---|---|---|---|
DcACO1 | 1 | 852 | 548 and 304 |
2 | 852 | 548 and 304 | |
DcACS1 | 1 | 1217 | 579 and 397 |
2 | 1217 | 728 and 489 | |
3 | 1217 | 802 and 415 | |
4 | 1217 | 883 and 334 | |
5 | 1217 | 893 and 324 |