Fig. 3
From: Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex
Protoplast developmental stages. a. First cell division at 5 days (× 20). b. multi-cellular division at 10 days (× 20). c. Microcalli formation at 14 days (× 20). d. DcACO1 transfected protoplast at 21 days old (× 10). e. DcACS1 transfected protoplast at 21 days old (× 10). Calli induction from protoplast-derived micro calli. f. non-transfected protoplast. g. DcACO1 transfected protoplast. h. DcACS1 transfected protoplast. Protoplast-derived calli on shoot induction media. i. non-transfected protoplast. j. DcACO1 transfected protoplast. k. DcACS1 transfected protoplast