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Table 3 RFP identification

From: Assessing data analysis techniques in a high-throughput meiosis-like induction detection system

Date

Thresholding

Polygonal gating

Percent red cells detected with quality thresholding + RFP and GFP

P-value from Wald test (compared to control)

Percent red cells detected with only GFP and RFP

P-value from Wald Test (compared to control)

Percent red cells detected with Polygonal gating

P-value from Wald Test (compared to control)

220502

1.78%

< 0.00001

1.82%

< 0.00001

2.2%

< 0.00001

220502

3.18%

< 0.00001

3.05%

< 0.00001

3.69%

< 0.00001

220502

33.71%

< 0.00001

30.19%

< 0.00001

38.64%

< 0.00001

220624

0.22%

0.00009

0.16%

0.01332

0.28%

< 0.00001

220624

0.76%

< 0.00001

0.89%

< 0.00001

1.18%

< 0.00001

220624

3.17%

< 0.00001

2.8%

< 0.00001

3.89%

< 0.00001

  1. Comparisons of RFP identification from thresholding for quality, RFP, and GFP; thresholding for only RFP and GFP; and polygonal gating