Table 2 Thresholding values based on mean fluorescence intensity values
From: Assessing data analysis techniques in a high-throughput meiosis-like induction detection system
Filter name | Filter | Thresholding for only RFP and GFP | Thresholding for quality, RFP, and GFP | ||||
|---|---|---|---|---|---|---|---|
RFP Gates | GFP Gates | Both Gates | RFP Gates | GFP Gates | Both Gates | ||
FSC.H | Forward scatter height | No threshold applied | No threshold applied | No threshold applied | > 55,000 | > 55,000 | > 55,000 |
FSC.H | Forward scatter area | No threshold applied | No threshold applied | No threshold applied | < 200,000 | < 200,000 | < 200,000 |
SSC.H | Side scatter height | No threshold applied | No threshold applied | No threshold applied | > 450 | > 450 | > 450 |
SSC.H | Side scatter height | No threshold applied | No threshold applied | No threshold applied | < 3500 | < 3500 | < 3500 |
Excitation/emission | |||||||
BV605.A | 407/602* | No threshold applied | No threshold applied | No threshold applied | > 800 | > 800 | > 800 |
BV510.A | 405/510* | No threshold applied | No threshold applied | No threshold applied | > 800 | > 800 | > 800 |
FITC.A | 494/520* | < 560 | > 560 | > 560 | < 560 | > 560 | > 560 |
FITC.A | 494/520* | N/A | < 10,000 | < 10,000 | N/A | < 10,000 | < 10,000 |
PE.CF594.A | 496,566/612* | > 1000 | < 1000 | > 1000 | > 1000 | < 1000 | > 1000 |
PE.CF594.A | 496,566/612* | < 10,000 | N/A | < 10,000 | < 10,000 | N/A | < 10,000 |