Fig. 5
From: Assessing data analysis techniques in a high-throughput meiosis-like induction detection system
Thresholding determination. Thresholding for only RFP and GFP (left). Thresholding for quality, RFP, and GFP (right). Colors represent all cells from an individual sample or a collation of multiple samples without RFP and GFP thresholds (10,000 maximum was applied). Red represents callus protoplasts from the RFP line collated from 05-02-2022 and 06-24-2022. Green represents callus protoplasts from the GFP line from 05-02-2022 and 06-24-2022. Gold represents callus protoplasts from GFP/RFP (Plant #10) genotype collated from 05-02-2022 and 06-24-2022. Purple represents callus protoplasts from GFP/RFP (Plant#12) genotype from 01-13-2022. Black represents callus protoplasts from the WT line from 01-13-2022. Black dotted lines represent the thresholds for RFP (PE.CF594.A; Horizontal) and the thresholds for GFP (FITC.A;Vertical) used for analysis