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Table 2 A. alba x A. numidica and P. nigra ET survival after 28 and 35 days of growth on proliferation medium, respectively

From: Cryopreservation of Abies alba × A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method

Species

Preculture

ET regrowth, %

Mean regrowth Time, days

A. alba x A. numidica

C

100.0 ± 0.00 a

7.0 ± 0.00 a

LN

84.4 ± 2.94 a

12.0 ± 0.63 b

P. nigra

C

100.0 ± 0.00 a

7.0 ± 0.00 a

S

100.0 ± 0.00 a

7.0 ± 0.00 a

SSG

100.0 ± 0.00 a

8.8 ± 1.75 a

LN

86.7 ± 13.33 a

17.9 ± 4.21 b

  1. C—Control—fresh ET, not cryopreserved; S—ET after treatment with sucrose (0.25–1.0 M) for 7 days; SSG—ET after treatment with sucrose (0.25–1.0 M) for 7 days and after 2 (A. alba x A. numidica) or 2.5 h (P. nigra) air desiccation over silica gel; LN—ET after stepwise desiccation, freezing and thawing from LN. Mean ± standard error; p ≤ 0.05; Tukey’s test
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Plant Methods

ISSN: 1746-4811