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Fig. 2 | Plant Methods

Fig. 2

From: Cryopreservation of Abies alba × A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method

Fig. 2

Logistic model of regrowth success of A. alba x A. numidica (a) and P. nigra (b) ETs during particular cryopreservation steps, C—Control—fresh ET, not cryopreserved; S—ET after treatment with sucrose (0.25–1.0 M) for 7 days; SSG—ET after treatment with sucrose (0.25–1.0 M) for 7 days and after air desiccation over silica gel (2 h in A. alba x A. numidica and 2.5 h in P. nigra); LN—ET after stepwise desiccation, freezing and thawing from LN

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