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Fig. 4 | Plant Methods

Fig. 4

From: Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)

Fig. 4

Silencing of miR319 in O. javanica using the CMV-based VbMS vector. A, Schematic representation of VbMS of miR319. B, Phenotypes of plants inoculated with CMV-STTM319 at 40 dpi. C, Systematic leaf morphology analysis of pCB301CMV-STTM319 in ‘Fq1’ (40 dpi). Scale bars are 2 cm. D, RT-PCR detection confirmed the expression of STTM319 in CMV-STTM319-infected plants. E, Stem-loop RT-qPCR analysis of miR319 expression levels in CMVâ–³2b- or CMV-STTM319-infected plants. F, Detection of the relative expression levels of the miR319 target genes TCP2 and TCP4

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