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Fig. 3 | Plant Methods

Fig. 3

From: Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)

Fig. 3

Silencing of the OjGID1 gene in O. javanica using the CMV VIGS vector. A, Construct diagram of infectious clones of pCB301CMV-OjGID1; B, RT‒PCR detection of GID1 of ‘Yzcbq’. C, Phenotypes of pCB301CMV-OjGID1 in ‘Yzcbq’ (30 dpi). Scale bars are 2 cm. D, Systematic leaf morphology analysis of pCB301CMV-OjGID1 in ‘Yzcbq’ (30 dpi). E Detection of pCB301CMV-OjGID1-infected O. javanica by agro-infection (30 dpi). F RT-qPCR analysis of OjGID1 expression levels in CMVâ–³2b- or CMV-OjGID1-infected plants. G RT-qPCR analysis of GA-related genes expression levels in CMVâ–³2b- or CMV-OjGID1-infected plants

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Plant Methods

ISSN: 1746-4811