Fig. 2
Silencing of the OjPDS gene in O. javanica using the CMV VIGS vector. A, Construct diagram of infectious clones of pCB301CMV-OjPDS. B, RT‒PCR detection of the PDSN and PDSC of O. javanica. C, Phenotypes of pCB301CMV-OjPDS in O. javanica (4 wpi). Scale bars are 2 cm. D, Detection of pCB301CMV-OjPDS-infected ‘Fq1’ by agro-infection (4 wpi). The vector plasmid with the CMV RNA3 insert was amplified as the positive (lane P) control. E, Detection of pCB301CMV-OjPDS-infected ‘Yzcbq’ by agro-infection (4 wpi). The vector plasmid with the CMV RNA3 insert was amplified as the positive (lane P) control