Table 4 Primer sets for verifying the BCA libraries

GTAAAACGACGGCCAGT (vector)

CAGGAAACAGCTATGAC (vector)

1-1

TTAAGGACACAAGGTGA

TCTTTATTTCACAAGCGGG

1-1 A (vector F + 1-1 R)

1-1 B (1-1 F + vector R)

1-2

CCTTGGAACCACCTACAGCT

ACCGTCGGATCACTAAGGC

1-2 A (vector F + 1-2 R)

1-2 B (1-2 F + vector R)

1-3

GGGAGGAGATGTAAGAAAAATTACCAA

TCGTCCACGGAGGGTGAG

1-3 A (vector F + 1-3 R)

1-3 B (1-3 F + vector R)

2-1

CGCGAGGGTGAGCTAAC

GGGTTGATCCTAAAGAGATACC

2-1 A (vector F + 2-1 R)

2-1 B (2-1 F + vector R)

2-2

GCTATATCCCCCCCGAG

CGGGGGCGCTCGTAGTG

2-2 A (vector F + 2-2 R)

2-2 B (2-2 F + vector R)

2-3

CCATAATGCCTTTCAAATCCTCC

CTCGCCTGCATGAAGCAG

2-3 A (vector F + 2-3 R)

2-3 B (2-3 F + vector R)

3-1

GAGTTGCTCTTTGGAGAGCAC

AATTGGCGTGCTTGAGG

3-1 A (vector F + 3-1 R)

3-1 B (3-1 F + vector R)

3-2

CTTCTAGGCAAACCTCCTGGT

AAGCAGGGGCACCTTGC

3-2 A (vector F + 3-2 R)

3-2 B (3-2 F + vector R)

3-3

CCATCTTTTCTTCAACTTGG

CGGATGTCAGCGGTTCG

3-3 A (vector F + 3-3 R)

3-3 B (3-3 F + vector R)

4-1

CGAGTATTTGAATACAGCGC

CAAACCAGAACCAGGCGG

4-1 A (vector F + 4-1 R)

4-1 B (4-1 F + vector R)

4-2

CTGACACAGCTTCTGAATG

GAAAGACAATTTGTCGCAC

4-2 A (vector F + 4-2 R)

4-2 B (4-2 F + vector R)

4-3

CGCCATTTCAAACCTGA

TCCCAATAGATGTGACCGA

4-3 A (vector F + 4-3 R)

4-4 B (4-3 F + vector R)