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Table 3 RecBCDase system

From: A novel in-situ-process technique constructs whole circular cpDNA library

Reagent

Volume (μL) per reaction

Final concentration

NEB (4)

5

1×

ATPs (10 mM)

15

3 mM

0.2% BSA

2.5

0.01%

RecBCDase

2

2 U

cpDNA (~ 20 ng/μL)

Variable

10 ng/μL

ddH2O

Variable

For 50 μL

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Plant Methods

ISSN: 1746-4811