Fig. 3
From: A novel in-situ-process technique constructs whole circular cpDNA library
The library of circular cpDNA. a The modified plasmid pBeloBAC11-MCS(+). The modified plasmid pBeloBAC11-MCS (+) has been inserted 18Â bp on the MCS of pBeloBAC11, adding two standard enzyme-digested sites PacI, PmeI that do not change the blue/white screening. b Assembly of the cpDNA libraries on a plate. Conducting the correct colonies from the selective mediums for the cpDNA library construction. A monolithically respectable BAC library is constructed with the circular cpDNA from the crops, marked Wheat by label 1 as 1-1, 1-2, 1-3; marked Maize by label 2, as 2-1, 2-2, 2-3; marked Soybean by label 3, as 3-1, 3-2, 3-3; marked Barley by label 4, as 4-1, 4-2, 4-3. The colony (Label 5-5) shows the blue after transforming the modified plasmid pBeloBAC11-MCS(+) into E. coil (DH10B). c Illustrations of the soybean circular cpDNA library. Cloneable soybean cpDNA of a suitable fragment inserted into the modifying vector for the formation of vector/insert recombinant DNA