Table 2 Genotype of vegetatively propagated plants (G1) at the target loci. Induced mutations are in bold

From: Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events

Clone	Genotype G0^a	Genotype G1^b
SH9B (P3–P6 = 983 bp; P3–P7 = 1919 bp; P3–P11 = 5101 bp)^c
T1–6 (P3–P7)	Het Del1066	Het Del1066
T1–29 (P3–P7)	Het Del1066	Het Del1066
T1–49 (P3–P7)	Het Del1066	Het Del1066 + R
T2–1 (P3–P6)	Het Del236	Het Del236
T2–3 (P3–P6)	WT	WT
T2–10 (P3–P6)	Hom Del236	Hom Del236
T2–12 (P3–P11)	Hom Del4288	Hom Del4288
T2–14 (P3–P7)	Hom Del1066	Hom Del1066
T2–15 (P3–P6)	WT	WT
T2–23 (P3–P6)	Het Del236	WT
T2–26 (P3–P6)	Het Del236 + Ins4	WT (Ins4)
T2–28 (P3–P6)	WT	WT
T2–29 (P3–P6)	Het Del236	Het Del236 + R
T2–30 (P3–P6)	WT	WT
T2–31 (P3–P6)	Hom Del236	Hom Del236
SH9B (G₅AT; C₁₁AA)^d
D1–48	AAT; C/TAA	AAT; TAA
D1–51	AAT; CAA	AAT; CAA
D1–69	AAT; C/TAA	AAT; C/TAA
D1–70	AAT; CAA	AAT; C/TAA
D1–84	AAT; TAA	AAT; TAA
D1–90	AAT; C/TAA	AAT; CAA
D1–93	AAT; C/TAA	AAT; C/TAA
D1–113	G/AAT; CAA	G/AAT; C/TAA
SH9B (G₂AG₄; G₅GC; TC₉T; C₁₁AA)^d
D2–9	GAG; GGC; TCT; CAA	GAG; GGC; TCT; CAA
D2–23	GAG; GGC; TCT; TAA	GAG; GGC; TCT; TAA
D2–27	GAG; GGC; TCT; C/TAA	GAG; GGC; TCT; C/TAA
D2–32	GAG; GGC; TCT; C/TAA	GAG; GGC; TCT; CAA
D2–33	GAG; GGC; TCT; CAA	GAG; GGC; TCT; CAA
D2–34	GAG; GGC; TCT; TAA	GAG; GGC; TCT; TAA
D2–38	GAG; GGC; TCT; CAA	GAG; GGC; TCT; CAA

^aG0, first-generation plants after regeneration
^bG1, Clones derived by tuber propagation of G0 plants, except for T2–1, T2–29, D1–70 and D1–113, which analysed progenies derived by several cycles of axillary bud propagation in vitro
^cT1 and T2 plants were analysed by PCR with primer pairs P3-P6, P3-P7 or P3-P11 (see Additional file 2: Fig. S2). The size of the PCR fragments in SH9B is indicated. The mutated genotype in the G0 and G1 generations is represented by the size (bp) of the deletion in homoplasmic (Hom) or heteroplasmic (Het) conditions. Ins4 in T2-26 stands for a 4 bp insertion into the largest WT amplicon, and R in T1-49 and T2-29 stands for novel bands putatively derived from mtDNA rearrangements
^dD1 and D2 plants were analysed by sequencing the fragment obtained by PCR with primers P4 and P5. The genotype of the selected codons in the two target windows is reported. Small numbers indicate the C/G positions in the sense strand

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ISSN: 1746-4811

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