From: Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples
Program | Step | Temperature ( °C) | Time | Number of cycles |
---|---|---|---|---|
1 | Initial denaturation | 94 | 10 min | 1× |
Denaturation | 94 | 30 s | 40× | |
Primer annealing | 54 | 40 s | ||
Extension | 72 | 1 min | ||
Final extension | 72 | 10 min | 1× | |
2 | Initial denaturation | 94 | 10 min | 1× |
Denaturation | 94 | 30 s | 40× | |
Primer annealing | 56 | 40 s | ||
Extension | 72 | 1 min | ||
Final extension | 72 | 10 min | 1× |