Fig. 5
From: A modified aeroponic system for growing small-seeded legumes and other plants to study root systems
Running the aeroponic system to observe M. truncatula symbiotic nitrogen fixation mutants. a Sections of the modified trash can lid are demarcated with lab tape. Individual M. truncatula seedlings can be seen in each of the sectors, on the day of sowing them into the aeroponic device. b M. truncatula seedlings 10 days after transfer to the aeroponic system, after 5 d of growth on full N and 5 d in N-starvation medium. c M. truncatula seedlings 25 d after transfer to the aeroponic system, 15 days post inoculation with S. meliloti. Individual mutants within sectors are visible, showing with chlorotic leaves (single arrow). One sector contains a homozygous Fix-genotype demonstrating stunted growth (double arrows). d The root system from the aeroponic system in panel c is easy to see after turning off the humidifier unit and lifting the lid of the modified trash can chamber. The arrow points to nodulated roots