Fig. 5

Analysis of transcriptome sequencing data. A Principal component analysis (PCA) analysis of transcriptome sequencing samples. B Upset plot of differentially expressed gene (DEGs) in different culture durations. The top column represents the DEGs’ number of intersections, and the left column graph represents the DEGs’ number of each dataset. Five separate blue dots represent the DEGs that are only present in one dataset. The DEGs present in at least two datasets are marked as connected blue dots. The connection represents the intersection, and the blue dots on the line represent the corresponding datasets. C The number of differentially expressed upregulated and downregulated genes between cultured and not cultured. D Gene Ontology (GO) enrichment analysis of DEGs between differentiation cultures at 1 and 0 days. E GO enrichment analysis of DEGs between differentiation cultures at 2 and 0 days. F GO enrichment analysis of DEGs between differentiation cultures at 3 and 0 days. G GO enrichment analysis of DEGs between differentiation cultures at 4 and 0 days. H GO enrichment analysis of DEGs between differentiation cultures at 5 and 0 days