Fig. 3From: Leaf-GP: an open and automated software application for measuring growth phenotypes for arabidopsis and wheatSteps of image pre-processing and calibration. a, b Fix the height (i.e. y-axis) of all processed images in a given series to reduce computational complexity, raw images are not affected. c Detect red circular markers as the image scale. d Extract ROI from the original image based on red markers’ positions. e Denoise the image to smooth leaf surface for the global leaf segmentationBack to article page