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Table 4 DNA yield and purity range obtained for all sample extracts using the Qiagen extraction method with and without using liquid nitrogen (N) in the extraction

From: Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize

#

Sample ID

User name

Nucleic acid conc.

Unit

A260

A280

260/280

260/230

Sample type

Factor

1

Probe 1_1

M10 1st elution

21.8

ng/µL

0.435

0.251

1.73

1.12

DNA

50.00

2

Probe 1′_1

M10 2nd elution

19.3

ng/µL

0.386

0.201

1.93

5.62

DNA

50.00

3

Probe 2_1

M321 1st elution

8.5

ng/µL

0.171

0.102

1.67

2.08

DNA

50.00

4

Probe 2′_1

M321 2nd elution

7.3

ng/µL

0.147

0.103

1.43

1.66

DNA

50.00

5

Probe 1_2

M10 1st elution, N

10.7

ng/µL

0.213

0.135

1.58

1.61

DNA

50.00

6

Probe 1′_2

M10 2nd elution

23.6

ng/µL

0.472

0.256

1.84

1.71

DNA

50.00

7

Probe 2_2

M321 1st elution, N

7.0

ng/µL

0.140

0.123

1.14

0.62

DNA

50.00

8

Probe 2′_2

M321 2nd elution, N

10.2

ng/µL

0.203

0.134

1.51

4.06

DNA

50.00