Fig. 4From: Use of the non-radioactive SUnSET method to detect decreased protein synthesis in proteasome inhibited Arabidopsis rootsRecovery of protein synthesis in roots after removal of plants from MG132. Plants were treated with or without MG132 for 4 h, after which plants were washed and placed into Hoagland’s media without MG132 for 0.5 and 3 h. a Immunoreactive proteins containing puromycin were detected on a 15 % SDS-PAGE gel. Smaller letters in parenthesis represent band intensity relative to control on dried membranes using image-J. Images are representative of two other experimental replicates. Membranes were stained with Ponceau Red prior to immunblotting. b Immunoblot of polyubiquitinated proteins and Cox2Back to article page