Reagent | Amount per liter |
---|---|
523 Medium (1 L)a | |
Sucrose | 10 g |
Casein enzymatic hydrolysate | 8 g |
Yeast extract | 4 g |
K2HPO4 anhydrous | 2 g |
MgSO4 anhydrous | 0.15 g |
Induction medium (recipe per 30 mL volume)b | |
Liquid ED (recipe described in [30]) | 30 mL |
0.1 M acetosyringone (Sigma Cat. # D134406) | 30 μL |
l-Proline | 0.00465 g |
20 % V8 Media (1 L)c | |
Bacto Agar | 15 g |
Calcium carbonate (CaCO3) | 3 g |
Campbell’s V8 Vegetable Juice | 200 mL |