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Fig. 6 | Plant Methods

Fig. 6

From: Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.

Fig. 6

Images representing stages in leaf transformation process. a Stage C cacao leaf with tip and base removed. Scale bar represents 1 cm. b Two halves of a cacao leaf placed into petri dishes with wet paper towel and filter paper. Scale bar represents 1 cm. c Ideal EGFP coverage seen 48 h after vacuum infiltration of leaves with Agrobacterium. Scale bar represents 1 mm. d Photograph of untransformed leaf tissue using GFP filter. Scale bar represents 1 cm

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Plant Methods

ISSN: 1746-4811