Fig. 5From: A method for detecting single mRNA molecules in Arabidopsis thaliana Automated image analysis of PP2A mRNA. a Representative maximum projection image of cell files labeled with PP2A mRNA probes (red). DNA labeled with DAPI (blue). b, c Screen shots showing sequential detection steps used to determine positive mRNA signals. d Cell segmentation, where a false-color is rendering individual cells. e Output image indicating the number of mRNA signals detected on each cell segmented in (d). Scale bar = 10 μmBack to article page