Fig. 4From: Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/CasReplicon-mediated GT. The replicon is released from the T-DNA, circularises at the LIRs and undergoes rolling circle replication. This leads to the promoter being positioned upstream of the nuclease ORF. Upon DSB-induction in the target locus, the integration of the donor sequence can be achieved by HRBack to article page