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Fig. 4 | Plant Methods

Fig. 4

From: A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard

Fig. 4

Transcriptional activation and modulation of the nopaline synthase promoter (pNOS). a Fluc/Rluc ratios obtained with dCas9:VP64 and dCas9:EDLL in combination with gRNAs 3, 4 and 5. b Comparison of the Fluc/Rluc ratios obtained for gRNAs 3, 4 and 5 in combination with the different dCas9 variants reported on this work. All values were normalized to the Fluc/Rluc ratios of the reference sample set as 1. Bars represent average values of three samples ± SD

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