Fig. 3
From: A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard
Transcriptional repression of the nopaline synthase promoter (pNOS) with different variants of the dead Cas9. a Schematic representation of the gRNA target positions on the pNOS. The gRNAs were selected in both sense and antisense strands. In parenthesis the 5′ position of each gRNA according to the pNOS transcription start site. b Comparison of the repression rates mediated by the different gRNAs combinations targeting the pNOS in combination with the dCas9. c Repression rates of the dCas9:BRD and dCas9:SRDX in combination with gRNAs targeting different positions upstream the pNOS TATA-box. d Influence of the presence of the BRD domain fused to the dCas9 on the repression levels induced by gRNAs 1, 2 and 4. All values were normalized to the Fluc/Rluc ratios of a reference sample set as 1. Bars represent average values of three samples ± SD