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Figure 4 | Plant Methods

Figure 4

From: Genetic transformation of western clover (Trifolium occidentale D. E. Coombe.) as a model for functional genomics and transgene introgression in clonal pasture legume species

Figure 4

Southern blot hybridisation of T. occidentale T 0 translines. Total genomic DNA was prepared from foliar tissues of independently transformed plants and a non-transformed wild type control. DNAs were digested with the restriction enzyme Eco RV and probed with a DIG-labelled fragment of the 3′ region of the gusA reporter gene. Lanes 1–11 transformed plants, lane 12 non-transformed control and lane 13 is the DIG labelled DNA Molecular Weight Marker II (Roche).

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