Figure 1
From: FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei
Expression pattern of GFP- and RFP-fusion proteins during pollen development. (A) MGH3p::MGH3-eGFP localizes in the generative cell nucleus (GCN) after the first pollen mitosis, and is strongly accumulated in the sperm cell nucleus (SCN) of mature pollen. ACT11p::H2B-mRFP is initially expressed in the GCN until late bicellular pollen (BCP). After the second pollen mitosis the expression of this transgene is down-regulated in the germline, and it becomes strongly expressed in the vegetative nucleus (VN). (B) Merged magnification of a mature pollen grain expressing both transgenes. (C) Population of mature pollen grains from double homozygous plants, confirming strong and stable expression of both transgenes. Scale bars: 10 μm.