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Figure 1 | Plant Methods

Figure 1

From: Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants

Figure 1

Generalized diagram of split root inoculation in M. truncatula. A plant with two main roots is created via our protocols and each root is placed in Perlite in a separate pot. One root is inoculated first (Root A) and the second root (Root B) is inoculated at a later time point. The plants continue to grow for 21 days after the second inoculation, at which point they are removed from the Perlite system and nodules on each root are counted.

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