Figure 3
From: Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system
Silencing and expression from the CLCrV   Agrobacterium  vector was comparable to the biolistic vector. A) New growth on cotton plants agro-inoculated with the CLCrVB and CLCrV:CP- lacked visible symptoms at 35 dpi. B) New growth on cotton plants agro-inoculated with CLCrVB and CLCrVA:ChlI showed a sectored loss of chlorophyll throughout the leaf at 35 dpi. C) Plant bombarded with the biolistic form of the vector used in (B) showed a similar pattern of silencing as the plant shown in (B) at 38 dpi. D) Overlay of GFP and brightfield images showed vein-delimited GFP expression (white arrowhead) in new growth of plants agro-inoculated with CLCrVB and CLCrVA:GFP at 28 dpi.